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In paper chromatography, why is it important to mark the ...

Sent to Job Experts February 20 04:36 PM

In paper chromatography, why is it important to mark the original line and component locations on the paper with pencil and not a pen or marker? And why is it important when the dry cylinder paper in the beakers, the spotted areas were above the solvent surface?

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February 20 9:06 PM (4 hours and 30 minutes and 4 seconds later)
         
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Question 1:
Pen and markers contain ink/pigments and they will seperate as components along with what you are tring to identify, contaminating the sample. Pencil graphite will not.

Question 2: I am not sure I understand the question the way you have it written but, the spot to be analyzed needs to be above the solution, on dry paper so the solution can "crawl" upward and catch each component in the sample. You are not "washing" it, you want the different components in the spot to be analysed to be drawn up through the paper.


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February 20 9:12 PM (5 minutes and 16 seconds later)
         
let me ask you this then, why are all calculated Rf values less than one?
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February 20 9:59 PM (47 minutes and 46 seconds later)
         
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Bethany -- business owner, Executive Director -- 100% Positive Feedback on 1 Job Accepts
Owned own PR business for 15 years, and am Executive Director of a non-profit
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